| Carattere | Attore |
|---|---|
| Jake Sully | Sam Worthington |
| Neytiri | Zoe Saldana |
| Colonnello Miles Quaritch | Stefano Lang |
| Dott.ssa Grazia Agostino | Sigourney Weaver |
| Trudy Chacon | Michelle Rodríguez |
| Tsu'tey | Laz Alonso |
| Parker Selfridge | Giovanni Ribisi |
| Norma Spellman | Joel David Moore |
| Dottor Max Patel | Dileep Rao |
| Eytukan | Wes Studi |
| Mo'at | CCH Pounder |